Lab === þ UA þ Urine Dipstick false + and false - þ Order of geting tubes: (Email fromMercy pathologist) > Today I was investigating a questionable PT result on a patient, and I > suspected that the correct order of draw may not have been followed. One > day the patient's PT result was > 90, and the next day it was 15. I > asked 2 different personnel in one of your departments what the correct > order of draw for a blue top, purple top, and green top tube would be. > Neither was aware that there was a required order of draw. Although I > can't prove that this was the problem with this patient's result, it > most likely was. > > The important fact to remember is that different tubes have different > substances in them that can interfere with results, if the tubes are not > drawn in the correct order. For example, purple top tubes contain EDTA > and green top tubes contain heparin, both of which will cause inaccurate > results for PT and PTT, if they get into the blue top tube. > > The correct order of draw is on page 11 of the lab manual, which can be > found at the Mercynet site: > > Additive > First: Pink/Red None > Blue Sodium citrate > Gold None, but contains a clot activator > Green Heparin > Lavender EDTA > Yellow Acid citrate dextrose > Dark blue None (used for metals) > Last: Gray Sodium fluoride þ Gaps: þ Tests of Hemostasis þ Bicarbonate Measurement on SMA: þ CO levels: þ Fecal occult blood (guaiac; hemoccult) þ Drawing Labs from an IV or Saline Lock: - If one stopped the IV infusion for 2 minutes before drawing the blood specimen the analysis of CBC and electrolytes did not differ from direct venipuncture except for an artifactual decrease in the serum HCO3 (and an increase in Glucose after the infusion of D5W). If a 5 cc aliqout of blood was discarded even glucose could be reliably assayed from a previously functioning IV site. The decrement in HCO3 was ascribed to the small amount of blood obtained relative to the size of the vacutainer tube which allowed CO2 to escape. Surely, what's good for recently discontinued IV lines is good for heplocks. --H. Louzon MD [Herr et. al. Intravenous Catheter Aspiration for Obtaining Basic Analytes During Intravenous Infusion. Ann Emer Med 1990;19:789-792.]