Bicarbonate Measurement ======================= þ Partially filled vacutainer tubes may cause falsely low HC03 levels. Author suggests correcting by adding 0.5-0.6 mmol/L to the HCO3 level and subtract 0.2-0.3 from the anion gap for every cc of air in the vacutainer. This has not been tested for acidotic patients. [Herr, RD. Pseudometabolic acidosis caused by underfill of vacutainer tubes. Ann Emerg Med 1992; 21:177.]